绱娓补鍎�鏂规硶鍘熺悊
鍦� pH 锛� 2 鐨勬浠朵笅锛屾ǎ鍝佷腑鐨勬补椤炵墿璩�(zh矛)琚宸辩兎钀冨彇锛岃悆鍙栨恫缍�(j墨ng)鐒℃按纭吀閳夎劔姘�锛屽啀缍�(j墨ng)閬庣閰搁巶鍚搁檮闄ゅ幓鍕曟鐗╂补绛夋サ鎬х墿璩�(zh矛)鍚�锛屼簬 225 nm 娉㈤暦铏曟脯瀹氬惛鍏夊害锛岀煶娌归鍚噺鑸囧惛鍏夊害鍊煎悎鏈椾集 - 姣旂埦瀹氬緥銆�
鍓嶈檿鐞嗘椹�
鎵嬪嫊钀冨彇鏂规硶
1.钀冨彇
灏囨按妯ｅ叏閮ㄨ綁(zhu菐n)绉昏嚦 1 000 mL 鍒嗘恫婕忔枟涓�锛岄噺鍙�25.0 mL 姝ｅ繁鐑锋礂婊岄噰妯ｇ摱鍚庯紝鍏ㄩ儴杞�(zhu菐n)绉昏嚦鍒嗘恫婕忔枟涓�锛屽厖鍒嗘尟鎼� 2 min锛屾湡闁撶稉(j墨ng)甯搁枊鍟熸棆濉炴帓姘�锛岄潨缃垎灞� 30 min 鍚�锛屽皣涓婂堡鐨勬湁姗熺浉閫�(j矛n)琛岃劔姘淬��
2.鑴按
锛�1锛夋(bi膩o)婧�(zh菙n)瑕�(gu墨)瀹氾細灏囦笂灞ゆ湁姗熺浉钀冨彇娑茶綁(zhu菐n)绉昏嚦宸插姞鍏�3 g鐒℃按纭吀閳夌殑閷愬舰鐡朵腑锛岃搵绶婄摱濉�锛屾尟鎼栨暩(sh霉)娆�锛岄潨缃�銆傝嫢鐒℃按纭吀閳夊叏閮ㄧ祼(ji茅)濉�锛岄渶瑁滃姞鐒℃按纭吀閳夌洿鑷充笉鍐嶇祼(ji茅)濉娿��
3.鍚搁檮
妯�(bi膩o)婧�(zh菙n)瑕�(gu墨)瀹氾細
绻肩簩(x霉)鍚戣悆鍙栨恫涓姞鍏�3 g纭呴吀閹�锛岀疆浜庢尟钑╁櫒涓�锛屼互 180~220 r/min 鐨勯�熷害闇囪暕 20 min锛岄潨缃矇娣�銆傚湪鐜荤拑婕忔枟搴曢儴澧婁笂灏戦噺鐜荤拑妫夛紝閬庢烤锛屽緟娓�銆�
钀冨彇娉ㄦ剰浜嬮爡锛�
锛�1锛夋(bi膩o)婧�(zh菙n)涓湭瑕�(gu墨)瀹氳悆鍙栨檪浜哄伐鎸悥鐨勬柟鍚�銆侀牷鐜囥�佸箙搴︾瓑鍙冩暩(sh霉)锛屽缓璀伴�氶亷瑕�(gu墨)鑼冩尟鎼栫窗(x矛)绡�(ji茅)浠ユ笡灏戜汉鍝℃搷浣滄墜娉曚笉鍚岄�犳垚鐨勮宸�銆�
渚嬪: 鍦ㄤ汉宸ユ尟鎼栧墠鍏堟鏌ョ摱濉炴槸鍚﹀绶娿�佹斁姘撮枼鏄惁宸查枆鍚�;灏囧垎娑叉紡鏂楁┇缃悗闆欐墜鎸佺穵锛屼互鑲橀棞(gu膩n)绡�(ji茅)鐐烘敮榛炲仛涓婁笅鏂瑰悜鎸悥锛岄牷鐜囪嚦灏� 120 娆�/min锛岀偤閬垮厤婕忔恫涓嶅缓璀板乏鍙虫尟鎼�;鎸悥 1 min 鍚庢斁姘� 1 娆�锛屽啀鎸悥 1 min 鍚�锛岄潨缃�銆�
锛�2锛夎悆鍙栨恫鍑虹従(xi脿n)涔冲寲鐝�(xi脿n)璞℃檪锛屽彲婊村姞 3 锝� 4 婊寸劇姘翠箼閱囩牬涔�銆�
涓�銆佹帶鍒跺洜绱犲垎鏋�
绱娉曢噰鐢ㄦ宸辩兎浣滅偤妯ｅ搧钀冨彇婧跺姂锛屾(bi膩o)婧�(zh菙n)涓矑鏈夌洿鎺ヨ(gu墨)瀹氭宸辩兎鐨勭磾搴�锛岃�屾槸灏嶆憾鍔戠殑閫忓厜鐜囧仛浜嗚姹傦紝鎵�浠ヤ笉璜栦娇鐢ㄧ殑浣曠ó绱斿害鐨勬宸辩兎锛屽垎鏋愭ǎ鍝佸墠鍧囬渶瑕佸皪浣跨敤鐨勬宸辩兎鍋氶�忓厜鐜囨椹�銆�
1.姝ｅ繁鐑风殑妾㈡脯
婧跺姂姝ｅ繁鐑风殑妾㈤鏄按璩�(zh矛)鐭虫补椤炴娓殑绗竴姝ュ拰淇濊瓑锛屾柊妯�(bi膩o)婧�(zh菙n)涓皪灏嶆宸辩兎閫忓厜鐜囩殑妾㈤鍜岃劔鑺宠檿鐞嗗仛鍑轰簡瑕佹眰锛�
浣跨敤鍓嶄簬娉㈤暦 225 nm铏曪紝浠ユ按鍋氬弮姣旀脯瀹氶�忓厜鐜�锛岄�忓厜鐜囧ぇ浜�90%鏂瑰彲浣跨敤锛屽惁鍓囬渶鑴姵铏曠悊銆傝劔鑺宠檿鐞嗘柟娉曪細灏�500 ml 姝ｅ繁鐑峰姞鍏�1 000 ml 鍒嗘恫婕忔枟涓�锛� 鍔犲叆25 ml纭吀钀冩礂10 min锛屾鍘荤～閰哥浉 閲嶅京(f霉)涓婅堪鎿嶄綔锛岀洿鑷崇～閰哥浉杩戠劇鑹�锛� 鍐嶇敤钂搁ぞ姘磋悆娲�3娆�锛岃嚦閫忓厜鐜囧ぇ浜�90%鍗冲彲銆�
妯�(bi膩o)婧�(zh菙n)涓殑瑕佹眰鏈夊叐闋呯洿鎺ュ奖闊挎宸辩兎閫忓厜鐜囩殑鎸囨(bi膩o)鏈綔鍑鸿(gu墨)瀹�锛屽嵆锛�
娓畾閫忓厜鐜囨墍浣跨敤鐨勫弮姣旀憾娑叉按鐨勭礆鍒ワ紱娓畾婧跺姂閫忓厜鐜囦娇鐢ㄧ殑姣旇壊鐨胯(gu墨)鏍�銆�
濮戜笖瑾�(r猫n)鐐哄椹楀鐢ㄦ按鍧囩偤瓒呯磾姘�
浠ュ椹楀瓒呯磾姘寸偤鍙冩瘮
閲囩敤25px鐭宠嫳姣旇壊鐨�锛屽競闈笂璩艰卜澶ч儴鍒嗚壊璀滅磾绱氭宸辩兎鍧囧彲鐩存帴閬�(d谩)鍒�90%閫忓厜鐜囪姹傦紝鐒￠渶閫�(j矛n)琛岃劔鑺宠檿鐞�锛岃�屽劒(y艒u)绱氱磾鍜屽垎鏋愮磾姝ｅ繁鐑峰墖闇�瑕侀��(j矛n)琛岃劔鑺宠檿鐞�锛�
閲囩敤50px鐭宠嫳姣旇壊鐨�锛屽競闈笂璩艰卜鐨勫绋父鐢ㄨ壊璀滅磾绱氭宸辩兎閮界劇娉曠洿鎺ラ仈(d谩)鍒�90%閫忓厜鐜囪姹�锛屽潎闇�瑕侀��(j矛n)琛岃劔鑺宠檿鐞�銆�
鎵�浠ユ(bi膩o)婧�(zh菙n)涓渶瑕侀��(j矛n)涓�姝ユ槑纰洪�忓厜鐜囨脯瀹氭墍浣跨敤鐨勬瘮鑹茬毧瑕�(gu墨)鏍�銆�
2.钀冨彇鏂瑰紡
妯�(bi膩o)婧�(zh菙n)涓(gu墨)瀹氾細灏囨ǎ鍝佸叏閮ㄨ綁(zhu菐n)绉昏嚦1000 ml鍒嗘恫婕忔枟涓紝閲忓彇25.0 ml姝ｅ繁鐑锋礂婊岄噰妯ｇ摱鍚�锛屽叏閮ㄨ綁(zhu菐n)绉昏嚦鍒嗘恫婕忔枟涓�銆傚厖鍒嗘尟鎼�2 min锛屾湡闁撶稉(j墨ng)甯搁枊鍟熸棆濉炴帓姘�锛岄潨缃垎灞ゅ悗锛� 灏囦笅灞ゆ按鐩稿叏閮ㄨ綁(zhu菐n)绉昏嚦1000 ml閲忕瓛涓�锛屾脯閲忔ǎ鍝侀珨绌嶅苟瑷橀寗